期刊論文
| 學年 | 89 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2001-03-01 |
| 作品名稱 | Microbial reclamation of shellfish wastes for the production of chitinases |
| 作品名稱（其他語言） | |
| 著者 | Wang, San-lang; Hwang, Jau-ren |
| 單位 | 淡江大學生命科學研究所 |
| 出版者 | PA: Elsevier Inc. |
| 著錄名稱、卷期、頁數 | Enzyme and Microbial Technology 28(4-5), pp.376-382 |
| 摘要 | Shrimp and crab shell powder (SCSP), prepared by treating shellfish processing waste with boiling and crushing, was used as a substrate for isolating chitinolytic microorganisms. Three potential strains (E1, J1, and J1-1) were isolated and identified as Bacillus cereus, B. alvei, and B. sphaericus, respectively. Three extracellular chitinases (FB1, FB2, and FB3) were purified from the culture supernatants of Bacillus cereus E1, B. alvei J1, and B. sphaericus J1-1, respectively. The molecular weights of FB1, FB2, and FB3 were 71,000, 71,000, and 65,000, respectively, by SDS-PAGE. The pIs for FB1, FB2, and FB3 were 7.1, 7.2, and 7.4, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of FB1 were pH 9, 50°C, pH 7 to 10, and 70°C; those of FB2 were pH 9, 60°C, pH 5 to 9, and 70°C; and those of FB3 were pH 7, 50°C, pH 5 to 9, and 60°C. The activities of all enzymes were strongly inhibited by Hg2+ and completely inhibited by glutathione, dithiothreitol, and 2-mercaptoethanol. |
| 關鍵字 | Shrimp shell; Chitin; Chitinase; Bacillus cereus; Bacillus alvei; Bacillus sphaericus |
| 語言 | en |
| ISSN | 0141-0229 |
| 期刊性質 | 國外 |
| 收錄於 | SCI EI |
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| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/61862 ) |