期刊論文

標題 Microbial reclamation of shellfish wastes for the production of chitinases
學年 89
學期 2
出版(發表)日期 2001/03/01
作品名稱 Microbial reclamation of shellfish wastes for the production of chitinases
作品名稱(其他語言)
著者 Wang, San-lang; Hwang, Jau-ren
單位 淡江大學生命科學研究所
出版者 PA: Elsevier Inc.
著錄名稱、卷期、頁數 Enzyme and Microbial Technology 28(4-5), pp.376-382
摘要 Shrimp and crab shell powder (SCSP), prepared by treating shellfish processing waste with boiling and crushing, was used as a substrate for isolating chitinolytic microorganisms. Three potential strains (E1, J1, and J1-1) were isolated and identified as Bacillus cereus, B. alvei, and B. sphaericus, respectively. Three extracellular chitinases (FB1, FB2, and FB3) were purified from the culture supernatants of Bacillus cereus E1, B. alvei J1, and B. sphaericus J1-1, respectively. The molecular weights of FB1, FB2, and FB3 were 71,000, 71,000, and 65,000, respectively, by SDS-PAGE. The pIs for FB1, FB2, and FB3 were 7.1, 7.2, and 7.4, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of FB1 were pH 9, 50°C, pH 7 to 10, and 70°C; those of FB2 were pH 9, 60°C, pH 5 to 9, and 70°C; and those of FB3 were pH 7, 50°C, pH 5 to 9, and 60°C. The activities of all enzymes were strongly inhibited by Hg2+ and completely inhibited by glutathione, dithiothreitol, and 2-mercaptoethanol.
關鍵字 Shrimp shell; Chitin; Chitinase; Bacillus cereus; Bacillus alvei; Bacillus sphaericus
語言 英文
ISSN 0141-0229
期刊性質 國外
收錄於 SCI;EI
產學合作
通訊作者
審稿制度
國別
公開徵稿
出版型式