期刊論文

標題 Purification and characterization of a chitosanase from a nattokinase producing strain Bacillus subtilis TKU007
學年 96
學期 2
出版(發表)日期 2008/02/01
作品名稱 Purification and characterization of a chitosanase from a nattokinase producing strain Bacillus subtilis TKU007
作品名稱(其他語言)
著者 Wang, San-Lang; Yeh, Pe-Yi
單位 淡江大學化學學系
出版者 Camden: Elsevier Ltd
著錄名稱、卷期、頁數 Process Biochemistry 43(2), pp.132-138
摘要 An extracellular surfactant-stable chitosanase was purified from the culture supernatant of Bacillus subtilis TKU007 with shrimp shell wastes as the sole carbon/nitrogen source. The TKU007 chitosanase was suppressed by the nattokinase, which appeared at the 1st day. The molecular weight of TKU007 chitosanase was approximately 25 and 33 kDa determined by SDS-PAGE and gel filtration, respectively. The optimum pH, optimum temperature, pH stability and thermal stability of TKU007 chitosanase were pH 7, 37 °C, pH 4–9 and 37 °C, respectively. Closest similarity of TKU007 chitosanase was found on the 226th–229th amino acids of B. subtilis ywtD gamma-dl-glutamyl hydrolase. The amino acid sequence of TKU007 nattokinase was similar to that of B. subtilis nattokinase.
關鍵字 Bacillus subtilis; Chitosanase; Nattokinase; Surfactant stable; Shrimp shell wastes
語言 英文
ISSN 1359-5113
期刊性質 國外
收錄於 SCI;EI
產學合作
通訊作者
審稿制度
國別 美國
公開徵稿
出版型式