期刊論文

標題 Conversion of squid pen by Serratia ureilytica for the production of enzymes and antioxidants
學年 97
學期 1
出版(發表)日期 2009/01/01
作品名稱 Conversion of squid pen by Serratia ureilytica for the production of enzymes and antioxidants
作品名稱(其他語言)
著者 Wang, San-lang; Lin, Chin-lin; Liang, Tzu-wen; Liu, Kao-cheng; Kuo, Yi-hsuan
單位 淡江大學化學學系
出版者 Amsterdam: Elsevier BV
著錄名稱、卷期、頁數 Bioresource Technology 100(1), pp.316-323
摘要 Two proteases (P1 and P2) and a chitinase (C1) were purified from the culture supernatant of Serratia ureilytica TKU013 with squid pen as the sole carbon/nitrogen source. The molecular masses of P1, P2 and C1 determined by SDS–PAGE were approximately 50 kDa, 50 kDa and 60 kDa, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of P1, P2 and C1 were (pH 10, 40 °C, pH 7–11, and <50 °C), (pH 10, 40 °C, pH 8–11, and <40 °C) and (pH 6, 50 °C, pH 5–8, and <50 °C), respectively. P1 and P2 were inhibited by Mg2+, EDTA and C1 was inhibited completely by Cu2+. The antioxidant activity of TKU013 culture supernatant was 72% per mL (DPPH scavenging ability). With this method, we have shown that squid pen wastes can be utilized and have revealed its hidden potential in the production of functional foods.
關鍵字 Serratia ureilytica; Chitinase; Protease; Antioxidant activity; Squid pen wastes
語言 英文
ISSN 0960-8524
期刊性質 國外
收錄於 SCI;EI
產學合作
通訊作者 Wang, San-lang
審稿制度
國別 荷蘭
公開徵稿
出版型式 紙本