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標題
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Biodegradation of shellfish wastes and production of chitosanases by a squid pen-assimilating bacterium, Acinetobacter calcoaceticus TKU024 |
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學年
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100 |
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學期
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1 |
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出版（發表）日期
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2011/09/01 |
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作品名稱
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Biodegradation of shellfish wastes and production of chitosanases by a squid pen-assimilating bacterium, Acinetobacter calcoaceticus TKU024 |
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作品名稱（其他語言）
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著者
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Wang, San-Lang; Tseng, Wan-Nine; Liang, Tzu-Wen |
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單位
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淡江大學生命科學研究所; 淡江大學化學學系 |
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出版者
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Dordrecht: Springer Netherlands |
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著錄名稱、卷期、頁數
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Biodegradation 22(5), pp.939-948 |
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摘要
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Two chitosanases (CHSA1 and CHSA2) were purified from the culture supernatant of Acinetobacter calcoaceticus TKU024 with squid pen as the sole carbon/nitrogen source. The molecular masses of CHSA1 and CHSA2 determined by SDS-PAGE were approximately 27 and 66 kDa, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of CHSA1 and CHSA2 were (pH 6, 50°C, pH 4–10, ＜90°C) and (pH 7, 60°C, pH 6–11, ＜70°C), respectively. CHSA1 and CHSA2 had broad pH and thermal stability. CHSA1 and CHSA2 were both inhibited by EDTA and were inhibited completely by 5 mM Mn2+. CHSA1 and CHSA2 degraded chitosan with DD ranging from 60 to 98%, and also degraded some chitin. The most susceptible substrate was 60% deacetylated chitosan. Furthermore, TKU024 culture supernatant (1.5% SPP) incubated for 5 days has the most reducing sugars (0.63 mg/ml). With this method, we have shown that shellfish wastes may have a great potential for the production of bioactive materials. |
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關鍵字
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Chitosanase;Squid pen wastes;Acinetobacter calcoaceticus;Reducing sugar |
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語言
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英文(美國) |
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ISSN
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1572-9729;0923-9820 |
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期刊性質
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國外 |
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收錄於
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SCI |
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產學合作
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通訊作者
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Wang, San-Lang |
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審稿制度
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是 |
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國別
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公開徵稿
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出版型式
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電子版;紙本 |