標題
|
Biodegradation of shellfish wastes and production of chitosanases by a squid pen-assimilating bacterium, Acinetobacter calcoaceticus TKU024 |
學年
|
100 |
學期
|
1 |
出版(發表)日期
|
2011/09/01 |
作品名稱
|
Biodegradation of shellfish wastes and production of chitosanases by a squid pen-assimilating bacterium, Acinetobacter calcoaceticus TKU024 |
作品名稱(其他語言)
|
|
著者
|
Wang, San-Lang; Tseng, Wan-Nine; Liang, Tzu-Wen |
單位
|
淡江大學生命科學研究所; 淡江大學化學學系 |
出版者
|
Dordrecht: Springer Netherlands |
著錄名稱、卷期、頁數
|
Biodegradation 22(5), pp.939-948 |
摘要
|
Two chitosanases (CHSA1 and CHSA2) were purified from the culture supernatant of Acinetobacter calcoaceticus TKU024 with squid pen as the sole carbon/nitrogen source. The molecular masses of CHSA1 and CHSA2 determined by SDS-PAGE were approximately 27 and 66 kDa, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of CHSA1 and CHSA2 were (pH 6, 50°C, pH 4–10, <90°C) and (pH 7, 60°C, pH 6–11, <70°C), respectively. CHSA1 and CHSA2 had broad pH and thermal stability. CHSA1 and CHSA2 were both inhibited by EDTA and were inhibited completely by 5 mM Mn2+. CHSA1 and CHSA2 degraded chitosan with DD ranging from 60 to 98%, and also degraded some chitin. The most susceptible substrate was 60% deacetylated chitosan. Furthermore, TKU024 culture supernatant (1.5% SPP) incubated for 5 days has the most reducing sugars (0.63 mg/ml). With this method, we have shown that shellfish wastes may have a great potential for the production of bioactive materials. |
關鍵字
|
Chitosanase;Squid pen wastes;Acinetobacter calcoaceticus;Reducing sugar |
語言
|
英文(美國) |
ISSN
|
1572-9729;0923-9820 |
期刊性質
|
國外 |
收錄於
|
SCI |
產學合作
|
|
通訊作者
|
Wang, San-Lang |
審稿制度
|
是 |
國別
|
|
公開徵稿
|
|
出版型式
|
電子版;紙本 |