期刊論文

標題 A Novel Compound with Antioxidant Activity Produced by Serratia ureilytica TKU013
學年 101
學期 1
出版(發表)日期 2012/08/28
作品名稱 A Novel Compound with Antioxidant Activity Produced by Serratia ureilytica TKU013
作品名稱(其他語言)
著者 Kuo, Yao-Haur; Hsu, Hsiu-Ching; Chen, Yu-Chi; Liang, Tzu-Wen; Wang, San-Lang
單位 淡江大學生命科學研究所
出版者 Washington, DC: American Chemical Society
著錄名稱、卷期、頁數 Journal of Agricultural and Food Chemistry 60(36), pp.9043-9047
摘要 The secondary metabolites from the cultured supernatant of Serratia ureilytica TKU013 with squid pen as the sole carbon/nitrogen source were isolated and ascertained the mechanism of biological activity. The EtOAc layer, which has high DPPH scavenging activity, was applied to silica gel column chromatography with a gradient of CH2Cl2/MeOH solvent system, to yield A–H and MeOH fractions. The DPPH scavenging activity and cytotoxic activities against Doay and HEp-2 cell lines of these fractions were examined. The active fractions were further applied to purification by RP-HPLC, to obtain seven compounds, including a novel compound, serlyticin-A (1), together with six known compounds, serranticin (2), serratamolide A (3), thymine (4), (4-hydroxyphenyl)acetic acid (5), methyl p-hydroxybenzoate (6), and uracil (7). Their structures were determined by physical and extensive spectral analyses such as 1D and 2D NMR data, as well as comparison with literature values. Furthermore, the major secondary metabolites of EtOAc extract of the cultured supernatant were examined by the fingerprinting data of the HPLC system.
關鍵字 Serratia ureilytica;serlyticin-A;squid pen;antioxidant activity;cytotoxicity;DPPH
語言 英文(美國)
ISSN 1520-5118
期刊性質 國外
收錄於 SCI;
產學合作
通訊作者 王三郎
審稿制度
國別 美國
公開徵稿
出版型式 ,電子版,紙本