期刊論文
| 學年 | 101 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2012-10-15 |
| 作品名稱 | Fermented and enzymatic production of chitin/chitosan oligosaccharides by extracellular chitinases from Bacillus cereus TKU027 |
| 作品名稱（其他語言） | |
| 著者 | Wang, San-Lang; Liu, Chin-Pei; Liang, Tzu-Wen |
| 單位 | 淡江大學生命科學研究所; 淡江大學化學學系 |
| 出版者 | Kidlington: Pergamon |
| 著錄名稱、卷期、頁數 | Carbohydrate Polymers 90(3), pp.1305–1313 |
| 摘要 | Two chitinases, Chi I and Chi II, were purified from the culture supernatant of Bacillus cereus TKU027 with shrimp head powder (SHP) as the sole carbon/nitrogen source. The molecular masses of Chi I and Chi II determined using SDS–PAGE were approximately 65 kDa and 63 kDa, respectively. Chi I toward various surfactants showed high stability, such as SDS, Tween 20, Tween 40 and Triton X-100, and these surfactants were stimulator of Chi I chitinase activity. Concomitant with the production of Chi I and Chi II, chitin oligosaccharides were also observed in the culture supernatant, including chitobiose, chitotriose, chitotetrose and chitopentose at concentrations of 0.44 mg/mL, 0.08 mg/mL, 0.09 mg/mL and 0.43 mg/mL, respectively. Chitosan with 60% deacetylation was degraded by TKU027 crude enzyme to prepare chitooligosaccharides. MALDI-TOF MS analysis of the enzymatic hydrolyzates indicated that the products were mainly chitooligosaccharides with degree of polymerization (DP) in the 4–9 range. |
| 關鍵字 | Chitinase;Chitin oligosaccharides;Ahrimp heads;Bacillus cereus;Fermented;Enzymatic hydrolysis |
| 語言 | en |
| ISSN | 1879-1344 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | 王三郎 |
| 審稿制度 | 是 |
| 國別 | GBR |
| 公開徵稿 | |
| 出版型式 | ,電子版,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/79672 ) |