期刊論文
| 學年 | 101 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2012-10-01 |
| 作品名稱 | Caffeine treatment disturbs the angiogenesis of zebrafish embryos |
| 作品名稱（其他語言） | |
| 著者 | Yeh, Chien-Hung; Liao, Yun-Feng; Chang, Chao-Yuan; Tsai, Jen-Ning; Wang, Yun-Hsin; Cheng, Chien-Chung; Wen, Chi-Chung; Chen, Yau-Hung |
| 單位 | 淡江大學生命科學研究所; 淡江大學化學學系 |
| 出版者 | New York: Informa Healthcare |
| 著錄名稱、卷期、頁數 | Drug and Chemical Toxicology 35(4), p.361-365 |
| 摘要 | Caffeine is a widely consumed substance that occurs in numerous dietary sources, but teratogenic effects of caffeine intake during embryonic development are still not clear. In the present study, we used the zebrafish as a model to assess caffeine-induced toxicity on embryonic vascular development. A green fluorescent vascular endothelium transgenic line, Tg(fli1:egfp), was utilized for the sensitive detection of vascular development, including vasculo- and angiogenesis. Caffeine-treated embryos showed no defects in vasculogenesis, but revealed dose-dependent (250-350 ppm) developmental defects in intersegmental vessels, dorsal longitudinal anastomotic vessels, and subintestinal vein sprouting. Further, real-time polymerase chain reaction analysis of caffeine-treated embryos showed an upregulation of nrp1a along with a downregulation of sema3aa and sema3c. In conclusion, caffeine treatment induces defects of angiogenesis in zebrafish embryos. |
| 關鍵字 | Angiogenesis;caffeine;fluorescent protein;transgenic fish;zebrafish |
| 語言 | en_US |
| ISSN | 0148-0545 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Chen, Yau-Hung |
| 審稿制度 | 是 |
| 國別 | USA |
| 公開徵稿 | |
| 出版型式 | ,電子版 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/79660 ) |