期刊論文
學年 | 99 |
---|---|
學期 | 1 |
出版(發表)日期 | 2011-01-01 |
作品名稱 | Inductive properties of polypyridyl ruthenium complexes significantly regulate various protein distributions in Escherichia coli |
作品名稱(其他語言) | |
著者 | Ho, Ming-yi; Chiou, Min-lun; Du, Wan-shan; Chang, Fang-ying; Chen, Yau-hung; Weng, Yu-jong; Cheng, Chien-chung |
單位 | 淡江大學化學學系 |
出版者 | Philadelphia: Elsevier Inc. |
著錄名稱、卷期、頁數 | Journal of Inorganic Biochemistry 105(6), pp.902-910 |
摘要 | Ruthenium complexes with similar octahedral structures but different intrinsic inductive properties significantly influence the total cellular protein distributions, which may affect different metabolic pathways. A systematic study of the relationship between ruthenium complexes and Escherichia coli was undertaken, using two-dimensional gel electrophoresis analysis and the identification of various proteins by mass data mining. Based on the low similarities (<40%) between the total protein distributions, the inductive properties of the ruthenium complexes are relevant to the formation of the protein-Ru interaction in addition to the Ru-DNA interaction. Two major protein functions in E. coli BL21 that were reduced by compound 1 were oxidoreductases and transporters, corresponding to 29% and 25% of the 24 down-regulated proteins. The main biological processes of the proteins down-regulated by compound 1 were related to carbohydrate reactions, including in transport, tricarboxylic acid (TCA) cycle, glycolysis, and gluconeogenesis. All four ruthenium complexes shared similar up-regulated proteins, including clpB and kpyk1, and down-regulated similar proteins, including ompA and ybbN. This result supports that the presence of Ru-protein interactions is a major factor affecting bacteria growth, and particularly transport and carbohydrate-related reactions. |
關鍵字 | Ruthenium;2D gel;Polypyridyl;Metalloproteomic;LC/MS;Ru-protein interaction |
語言 | en |
ISSN | 0162-0134 |
期刊性質 | 國外 |
收錄於 | SCI |
產學合作 | |
通訊作者 | Cheng, Chien-chung |
審稿制度 | |
國別 | USA |
公開徵稿 | |
出版型式 | 紙本 |
相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/77225 ) |