期刊論文

標題 Molecular Cloning and Characterization of an Insecticidal Toxin from Pseudomonas taiwanensis
學年 99
學期 1
出版(發表)日期 2010/12/01
作品名稱 Molecular Cloning and Characterization of an Insecticidal Toxin from Pseudomonas taiwanensis
作品名稱(其他語言)
著者 Liu, Je-ruei; Lin, Yung-di; Chang, Shying-tzu; Zeng, Yi-fang; Wang, San-lan
單位 淡江大學化學學系
出版者 Washington: American Chemical Society
著錄名稱、卷期、頁數 Journal of Agricultural and Food Chemistry 58(23), pp.12343-12349
摘要 An insecticidal toxin gene, tccC, was cloned from the recently discovered novel species Pseudomonas taiwanensis using degenerate PCR and genomic walking. The DNA sequence of the tccC gene (2,940 bp) has an open reading frame encoding 980 amino acids with a calculated molecular weight of 107.93 kDa. The amino acid sequence alignment showed the highest sequence identity (41.2%) with the insecticidal toxin from Pseudomonas entomophila. To examine the insecticidal functionality of the tccC gene product, TccC was heterologously expressed in Escherichia coli as a recombinant His6 fusion protein and purified by immobilized metal ion-affinity chromatography. The recombinant TccC was fed to Drosophila larvae at a concentration of 350 ppm, which induced about 60% mortality within 72 h. The recombinant TccC was stable at pH 7.0 and at 37 °C. When the pH was less than 5.0 or greater than 9.0, or temperature was greater than 55 °C, less than 20% Drosophila larvae mortality was observed. These results prove that Pseudomonas taiwanensis could be used as a source for developing novel biopesticides.
關鍵字 Molecular cloning;insecticidal toxin;Pseudomonas taiwanensis
語言 英文
ISSN 0021-8561
期刊性質 國外
收錄於 SCI;EI
產學合作
通訊作者 Wang, San-lan
審稿制度
國別 美國
公開徵稿
出版型式 紙本