期刊論文
| 學年 | 99 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2011-04-01 |
| 作品名稱 | Embryonic exposure to diclofenac disturbs actin organization and leads to myofibril misalignment |
| 作品名稱（其他語言） | |
| 著者 | Chen, Ying-hsin; Chang, Chao-yuan; Wang, Yun-hsin; Wen, Chi-chung; Chen, Yun-chen; Hu, Sheng-chuan; Yu, Dah-shyong; Chen, Yau-hung |
| 單位 | 淡江大學生命科學研究所; 淡江大學數學學系 |
| 出版者 | Hoboken: John Wiley & Sons, Inc. |
| 著錄名稱、卷期、頁數 | Birth Defects Research Part B: Developmental and Reproductive Toxicology 92(2), pp.139-147 |
| 摘要 | The objective of this study was to investigate the embryotoxicity of diclofenac. Zebrafish (Danio rerio) embryos at 12 hpf were treated with different dosages of diclofenac (0–2,000 ppm) for different time courses (12–72 hr). Results showed no evident differences in survival rates or morphological changes between the mock-treated control (0 ppm) zebrafish embryos and those with 1-ppm diclofenac-exposure (12–24, 12–36 hpf). In contrast, after higher doses (5 and 10 ppm) of exposure, embryos displayed some defective phenotypes, including malformed somite boundary, a twisted body axis, and shorter body length. In addition, diclofenac-treated embryos exhibited significantly reduced frequencies of spontaneous in-chorion contractions in comparison with mock-control littermates (mock-control: 13.20±2.24 vs. 5–10 ppm diclofenac: 6.66±1.35–3.03±1.84). Subtle changes were easily observed by staining with specific monoclonal antibodies F59 and phalloidin to detect morphological changes in muscle fibers and formation of F-actin, respectively. Our data show that diclofenac treatment disturbs actin organization and muscle fiber alignment, thus causing malformed somite phenotypes. |
| 關鍵字 | actin;diclofenac;myotoxic;myod;zebrafish |
| 語言 | en_US |
| ISSN | 1542-9733; 1542-9741 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Chen, Yau-hung |
| 審稿制度 | |
| 國別 | USA |
| 公開徵稿 | |
| 出版型式 | 紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/77041 ) |