期刊論文
| 學年 | 90 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2002-01-01 |
| 作品名稱 | Functional analysis of the proximal promoter regions of fish rhodopsin and myf-5 genes using transgenesis |
| 作品名稱（其他語言） | |
| 著者 | 陳曜鴻; Chen, Yau-hung |
| 單位 | 淡江大學生命科學研究所 |
| 出版者 | |
| 著錄名稱、卷期、頁數 | Marine Technology 4, pp.247-255 |
| 摘要 | Little is known about the cis-acting elements controlling transcriptional activities of fish rhodopsin and myf-5 genes. Transgenic medaka and zebrafish were used to characterize promoters of carp (Cyprinus carpio) rhodopsin gene (cRh) and zebrafish (Danio rerio) myf-5 gene (myf-5), respectively. Transgenic medaka carrying different lengths of cRh upstream fragments fused with enhanced green fluorescence protein (EGFP) gene revealed several functional regions. Both upstream regions from ?1261 to ?163 bp (?1261/?163) and ?163/?138 contributed to enhance the retina-specific activities of cRh. The cNRE (?75/?63) and CSE (?52/?46) motifs located at the cRh proximal promoter were sufficient to drive the transgene expressed in retinae. The ?73/?68 within cNRE was also proved to be essential for the integrity of the minimal cis-regulatory elements. Regarding the myf-5, transgene expression in zebrafish embryos injected with different deletion fragments of zebrafish myf-5 upstream sequences showed that the ?2776/?2456 and ?1046/?844 regions contributed to the enhancer function. The ?290/?154 region might be involved in controlling the translocation of progenitor muscle cells. Moreover, the upstream ?82/?1 region was identified as a minimal promoter required for somite-specific expression. The ?82/?62 region was the most critical sequence for myf-5 specificity. |
| 關鍵字 | |
| 語言 | en_US |
| ISSN | 0025-3316 |
| 期刊性質 | 國外 |
| 收錄於 | |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 否 |
| 國別 | USA |
| 公開徵稿 | |
| 出版型式 | ,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/61854 ) |